The women from Sydney's inner west called it quits about 6:00am AEST after beating the previous record of 86 hours held by Dutchman Efraim van Oeveren.
The feat still needs to be verified by officials from Guinness World Records, but organisers are confident the record will be recognised.
The sisters, self-professed soccer fanatics, outlasted two other couch potatoes: Tim Chan and Joel Martinot.
In between live World Cup matches, most of which are broadcast in the early hours of the morning, the competitors had a choice of watching recorded matches or soccer documentaries.
They were monitored by a team of officials who ensured the girls did not take their eyes off the screen for more than five seconds.
They were allocated one five-minute break every hour and were allowed no more than five cups of coffee per day.
The Argyrou sisters are now at home having a well-deserved rest.
